'Growing Fungi and Bacteria of Plants\r'

'Introduction on that point are both sultry and non-virulent bacteria and kingdom Fungi that grow on limits. It is difficult to distinguish between the two without seemly inspection and diagnosis of the diseased plant to get by whether the bacteria or the fungus in question is the virulent or non-virulent one. Therefore pure cultures need to be isolated to know with absolute certainty which is the precipitating(prenominal) bacteria or fungus. Potato dextroglucose nutrient nutrient nutrient agar is a good nutrient agar for mycelia to expand on which is present in most fungous moulds. Standard nutrient agar is a usual utility used for non-fastidious microorganisms. 2 Aim The beat back is to isolate fungi and bacteria colonies from diseased and levelheaded leaves. Materials and Methods Materials used for the experiment was two of each: warning nutrient agar plate and potato dextrose agar plate. To remove any epiphytic or saprophytic microbes from the plant surface the thu mb is superficially sterilized with 100% ethanol. A scalpel to cut the pitchSterile body of irrigate for macerating the leaves. To isolate the fungi: Cut 5 pieces of leaf from the diseased leaf around the edges of the diseased theater so it contains both ruddy and diseased parts, fix it on the one potato dextrose agar plate. Then cut 5 pieces from the healthy leaf and place them on the second dextrose agar plates. To isolate bacteria: Macerate both the healthy and diseased leaves separately in the sterile water and streak it onto the two separate standard nutrient agar plate.Incubate all four plates at 25? C for up to 7 days. Discussion There is evolution on both the healthy and diseased agar plates for both bacteria and fungi. For the healthy fungous plate there is a single character reference of growth which raises that it is a natural non-pathogenic fungus that grows on plants. On the diseased plants there are 5 opposite colonies of fungus from the 5 different pieces of leaves. The one area has a clear zone where the fungus has inhibited the growth of the a nonher(prenominal) colonies.On the healthy bacterial plate there is rough bacterial growth which can also suggest non-pathogenic bacteria that grows on plants. On the diseased plant bacterial plate there is some bacterial growth but not a dowry and still fungal growth. Conclusion The lack of bacterial colonies on the bacterial plate and the fungal growth on the same plate suggests that the disease on the plant is caused by a fungal pathogen and not a bacterial one. There are fungi and bacteria that grow on plants that are not harmful towards the plant. ?\r\n'